The High-Throughput Genomics (HTG) Shared Resource supports whole genome sequencing on both the Illumina NovaSeq and the Illumina HiSeq 2500. The NovaSeq provides a cost-effective solution to sequence genomic DNA libraries constructed with kits that include PCR amplification of the library, whereas PCR-free libraries are best to sequence on the HiSeq 2500. The HTG Shared Resource supports multiple options for the construction of genomic DNA sequencing libraries. Library preparation options along with guidelines for DNA sequencing projects are described on the pages below:

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High-Throughput Genomics Director
Brian K. Dalley, PhD


HCI Senior Director Oversight
Alana Welm, PhD

Faculty Advisory Committee Chair
Katherine Varley, PhD

Faculty Advisory Committee Members
Richard Clark, PhD
Jason Gertz, PhD
Christopher Gregg, PhD
Mei Koh, PhD
Philip Moos, PhD
Andrew Post, MD, PhD
Sean Tavtigian, PhD
Joseph Yost, PhD