Overview

The High-Throughput Genomics (HTG) Shared Resource supports whole genome sequencing on both the Illumina NovaSeq and the Illumina HiSeq 2500. The NovaSeq provides a cost-effective solution to sequence genomic DNA libraries constructed with kits that include PCR amplification of the library, whereas PCR-free libraries are best to sequence on the HiSeq 2500. The HTG Shared Resource supports multiple options for the construction of genomic DNA sequencing libraries. Library preparation options along with guidelines for DNA sequencing projects are described on the pages below:

• DNA Sequencing
• ChIP Sequencing
• DNA Methylation
• Exome Enrichment
• Custom Target Enrichment
• RNA Sequencing
• Small RNA Sequencing
• Sample Preparation

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