Molecular Diagnostics Digital PCR

In collaboration with ARUP Laboratories, the Molecular Diagnostics section of the BMP shared resource offers services on the BioRad QX200™ AutoDG™ Droplet Digital™ PCR System.

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR™) applications.

Benefits of the QX200 Droplet Digital PCR System

• Precise and sensitive digital PCR solution for a wide variety of applications
• Flexible digital PCR chemistry—optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
• Flexible assay setup—scalable for high sensitivity or high throughput
• Droplet partitioning by the QX200 Droplet Digital technology to reduce bias from amplification efficiency and PCR inhibitors
• Convenient assay design—standard curves are not required

The associated Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical and eliminating user-to-user variability.

Benefits of the Automated Droplet Generator

• Generates droplets for up to 96 ddPCR reactions
• Nanoliter droplet partitioning—reduces bias from amplification efficiency differences and PCR inhibitors
• HEPA-filtered enclosure to reduce potential contamination and allow the system to be placed in any lab environment

Applications of the QX200 Droplet Digital PCR System

• Cancer biomarker studies and copy number variation
• Pathogen detection
• Next generation sequencing library quantification
• Gene expression analysis
• Environmental monitoring
• Food testing

Assay Design

The system works with most two-color (HEX and FAM) TaqMan real-time PCR assays. EvaGreen can also be used as an alternative for dye-based ddPCR detection, gene expression analysis, or genotyping studies. The Molecular Diagnostics section has extensive experience designing and optimizing probe-based assays. Please contact the core to discuss further.

Bio-Rad Digital PCR Assays

ThermoFisher TaqMan Assays

Other Resources

Introduction to Digital PCR

BioRad QX200™ AutoDG™ Droplet Digital™ PCR System