Digital PCR

In collaboration with ARUP Laboratories, the Molecular Diagnostics section of the BMP shared resource offers services on the BioRad QX200™ AutoDG™ Droplet Digital™ PCR System.

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR™) applications.

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Benefits of the QX200 Droplet Digital PCR System

  • Precise and sensitive digital PCR solution for a wide variety of applications
  • Flexible digital PCR chemistry—optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup—scalable for high sensitivity or high throughput
  • Droplet partitioning by the QX200 Droplet Digital technology to reduce bias from amplification efficiency and PCR inhibitors
  • Convenient assay design—standard curves are not required

The associated Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical and eliminating user-to-user variability.

Benefits of the Automated Droplet Generator

  • Generates droplets for up to 96 ddPCR reactions
  • Nanoliter droplet partitioning—reduces bias from amplification efficiency differences and PCR inhibitors
  • HEPA-filtered enclosure to reduce potential contamination and allow the system to be placed in any lab environment

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
  • Pathogen detection
  • Next generation sequencing library quantification
  • Gene expression analysis
  • Environmental monitoring
  • Food testing

Assay Design

The system works with most two-color (HEX and FAM) TaqMan real-time PCR assays. EvaGreen can also be used as an alternative for dye-based ddPCR detection, gene expression analysis, or genotyping studies. The Molecular Diagnostics section has extensive experience designing and optimizing probe-based assays. Please contact the core to discuss further.

Bio-Rad Digital PCR Assays

ThermoFisher TaqMan Assays

Other Resources

Introduction to Digital PCR

BioRad QX200™ AutoDG™ Droplet Digital™ PCR System

Contact Us

BMP Shared Resource Director
Chris Fillmore, PhD

Biorepository Director
Chris Fillmore, PhD
chris.fillmore@hci.utah.edu
801-213-5787

Molecular Diagnostics Director
John O'Shea, PhD
john.oshea@hci.utah.edu
801-587-4406

Research Histology Director
Lindsey Fairbourn, HTL(ASCP)
lindsey.s.fairbourn@aruplab.com
801-213-4272

Governance

HCI Senior Director Oversight
Alana Welm, PhD

Medical Director
Eric Snyder, MD, PhD

Faculty Advisory Committee Chairs
Philip Bernard, MD
Joshua Schiffman, MD

Faculty Advisory Committee Members
Nicola Camp, PhD
Howard Colman, MD, PhD
Michael Deininger, MD, PhD
Randy Jensen, MD, PhD
Charles Murtaugh, PhD